Будь ласка, використовуйте цей ідентифікатор, щоб цитувати або посилатися на цей матеріал: http://dspace.tnpu.edu.ua/handle/123456789/13232
Назва: Biomineralization-related specialization of hemocytes and mantle tissues of the Pacific oyster Crassostrea gigas
Автори: Ivanina, Anna V.
Falfushynska, Halina I.
Beniash, Elia
Piontkivska, Helen
Sokolova, Inna M.
Бібліографічний опис: Biomineralization-related specialization of hemocytes and mantle tissues of the Pacific oyster Crassostrea gigas / Ivanina A.V. et. al. // Journal of Experimental Biology, 2017. Vol. 220, no. 18. P. 3209-3221
Дата публікації: 2017
Видавництво: The Company of Biologists Ltd.
Ключові слова: Gene expression
ion transport
matrix proteins
biomineralization
cell-cell interactions
hemocytes
mantle
bivalves
Короткий огляд (реферат): The molluscan exoskeleton (shell) plays multiple important roles including structural support, protection from predators and stressors, and physiological homeostasis. Shell formation is a tightly regulated biological process that allows molluscs to build their shells even in environments unfavorable for mineral precipitation. Outer mantle edge epithelial cells (OME) and hemocytes were implicated in this process; however, the exact functions of these cell types in biomineralization are not clear. Pacific oysters (Crassostrea gigas) were used to study differences in the expression profiles of selected biomineralization-related genes in hemocytes and mantle cells, and the functional characteristics of hemocytes such as adhesion, motility and phagocytosis. The specialized role of OME in shell formation was supported by high expression levels of the extracellular matrix (ECM) related and cell–cell interaction genes. Density gradient separation of hemocytes revealed distinct phenotypes based on the cell morphology, gene expression patterns, motility and adhesion characteristics. These hemocyte fractions can be categorized into two functional groups, i.e. biomineralization and immune response cells. Gene expression profiles of the putative biomineralizing hemocytes indicate that in addition to their proposed role in mineral transport, hemocytes also contribute to the formation of the ECM, thus challenging the current paradigm of the mantle as the sole source of the ECM for shell formation. Our findings corroborate the specialized roles of hemocytes and the OME in biomineralization and emphasize complexity of the biological controls over shell formation in bivalves.
Опис: DOI 10.1242/jeb.160861
URI (Уніфікований ідентифікатор ресурсу): http://dspace.tnpu.edu.ua/handle/123456789/13232
ISSN: 0022-0949
1477-9145
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